Evaluation of the Antioxidative Activities of Gamma-Oryzanol and Tocotrienol Rich Fractions Extracted from Rice Bran and Bran Oil

Aktarun Nahar, Md. Abbas Ali, Md. Shamsul Alam, Bijoy Maitra

Abstract

The current study determined the antioxidant activities of g-oryzanol rich fraction (OzF) and tocotrienol rich faction (TRF) derived from rice bran. Total phenolic content (TPC), total flavonoid content (TFC) and oryzanol content of OzF and TRF were examined. We explored hydrogen peroxide, hydroxyl radical, and 2, 2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging and reducing power assays. OzF and TRF's total antioxidant activity (TAA) were investigated using conventional procedure. Total antioxidant activity of OzF was 361.29±6.17 AAE mg/g and TRF was 273.88±5.31 AAE mg/g. Additionally, TPC and TFC of TRF (189.83±4.37 mg GAE/g and 97.64±0.22 CE mg/g respectively) were lower than those of OzF (256.34±7.68 mg GAE/g and 118.31±0.42 CE mg/g, respectively). DPPH, H2O2, hydroxyl radicals scavenging activities and reducing power were present in all concentrations of the extracts, the effects being dose-dependent. The current study provided crucial information for the development of food products with high antioxidant efficacy by utilizing TRF and OzF.

Conclusion

The study's antioxidative findings unequivocally establish that, fractions rich in g-oryzanol and tocotrienol have the power of scavenging or inhibiting free radicals, similar to a primary antioxidant reacting with free radicals. This could mitigate the harm that free radicals do to the body. Strong hydrogen-donating capabilities and efficaciousness as scavengers of H2O2 and other free radicals can be credited for the extracts' diverse antioxidant methods. In addition, the extracts get engaged in competition with DPPH radicals and inhibits the production of chromogen. As a result, OzF and TRF both exhibit strong antioxidant activity against a variety of in vitro oxidative structures, making them valuable as prospective dietary supplements as well as easily accessible sources of naturally occurring antioxidants. A compound's reducing capacity may be a useful predictor of its ability to exert antioxidant action and it is easily assumed that, these antioxidants will be able to prevent the development of lipid peroxides. According to the study, antioxidant-rich fractions' reducing capacity gets enhanced in proportion to concentration. As compared to tocotrienol rich fraction, all concentrations of g-oryzanol rich fraction displayed a strong potential to serve as an antioxidant; this is possibly because of the high phenolic content of the former, which was discovered through the analysis of the samples' bioactive phytochemicals. The samples are thought to be able to work competitively and synergistically against free radicals. Therefore, free radicals can be stabilized or neutralized by antioxidant-rich components before they damage cells.

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